DNA

Part:BBa_J119022:Design

Designed by: Caleb J. Carr   Group: Eckdahl Lab   (2011-10-19)


HPP Bsa J23100 promoter in pSB1A2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 15
    Illegal SpeI site found at 80
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 39
    Illegal NheI site found at 62
    Illegal SpeI site found at 80
    Illegal NotI site found at 6
    Illegal NotI site found at 87
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 15
    Illegal SpeI site found at 80
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 15
    Illegal SpeI site found at 80
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 22
    Illegal BsaI.rc site found at 73


Design Notes

Sticky ends must match only the destination sites and none else. Otherwise part will not work. Otherwise, J23100 is a common promoter, but the fore and aft tails must be unique to function properly.


Source

J23100 promoter with added sticky ends due to digestion with BsaI for insert into a destination vector for HPP construct problems.

References